Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Alkali Scientific Radiant™ Probe No-ROX qPCR Kit, 10,000 Reactions, (100 x 1mL)
Radiant™ Probe No-ROX qPCR Kit, 10,000 Reactions, (100 x 1mL)
Cayman Chemical CndIda genus qPCR asy 1ea
The primers and probes in the Candida genus kit are provided in tube 1 as a 20X concentrated working solution. This is a single assay that will detects 29 species of Candida including Candida albicans, Candida auris, Candida dubliniensis, Candida glabrata, Candida guiliermondii, Candida krusei, Candida lusitaniae, Candida parapsilosis, Candida tropicalis Organism Type: Fungi Controls (recommended): CrAssphage, Human RPP30-DNA Enzyme Master Mixes (recommended): Empirical qPCR mastermix, Standard qPCR enzyme mixture (Item No. 40705) Panels: Fungi, GI, Nail, Ophthalmology, UTI, Vaginal microbiota, Wound Colors: Cy5 (Candida genus), HEX (RPP30) Instruments: Any instrument
ACROBiosystems E1A resDNA Quantitation Kit (qPCR)
Residual host cell DNA refers to fragments of DNA from the host organism that are left behind after a biological process such as the production of a biopharmaceutical product or the cultivation of a cell line. This residual DNA can potentially contaminate the final product and affect its safety and efficacy. In the context of biopharmaceutical production, regulatory agencies such as the FDA and EMA have set limits on the amount of residual host cell DNA that is acceptable in a final product. These limits vary depending on the type of product and the route of administration, and residue host cell DNA quantitative kits are designed to ensure that the final product is safe for human use.
ACROBiosystems HEK293 resDNA Quantitation Kit (qPCR)
Residual host cell DNA refers to fragments of DNA from the host organism that are left behind after a biological process such as the production of a biopharmaceutical product or the cultivation of a cell line. This residual DNA can potentially contaminate the final product and affect its safety and efficacy. In the context of biopharmaceutical production, regulatory agencies such as the FDA and EMA have set limits on the amount of residual host cell DNA that is acceptable in a final product. These limits vary depending on the type of product and the route of administration, and residue host cell DNA quantitative kits are designed to ensure that the final product is safe for human use.
Mayflower Bioscience HOTFIREPol EvaGreen qPCR ROX
HOT FIREPol Probe qPCR Mix Plus (ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol Probe qPCR Mix Plus (ROX) is optimized for DNA hydrolysis probes based on the 5´ flap endonuclease activity. HOT FIREPol DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
NEW ENGLAND BIOGROUP LUNA 2nd General Reusable Slide, Counts 1 sample at a time, Contains LUNA reusable slide and one glass coverslip, For brightfield and fluorescent cell counts.
LUNA 2nd General Reusable Slide. Counts 1 sample at a time. Contains LUNA reusable slide and one glass coverslip. Can be used for brightfield and fluorescent cell counts. One year warranty
New England Biolabs, Inc. LunaScript RT SuperMix - 2500 rxns
LunaScript RT SuperMix is an optimized master mix containing all the necessary components for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow. It features the thermostable Luna Reverse Transcriptase which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is included to protect template RNA from degradation. LunaScript RT SuperMix contains random hexamer and poly-dT primers allowing even coverage across the length of the RNA targets. In addition LunaScript RT SuperMix contains dNTPs and a blue tracking dye providing a visual indicator that can be followed throughout the two-step RT-qPCR process. This product offers robust linear and sensitive detection using total RNA inputs as high as 1 g and as low as single copies of RNA.
New England Biolabs, Inc. LunaScript RT SuperMix - 100 rxns
LunaScript RT SuperMix is an optimized master mix containing all the necessary components for first strand cDNA synthesis in the context of a two-step RT-qPCR workflow. It features the thermostable Luna Reverse Transcriptase which supports cDNA synthesis at elevated temperatures. Murine RNase Inhibitor is included to protect template RNA from degradation. LunaScript RT SuperMix contains random hexamer and poly-dT primers allowing even coverage across the length of the RNA targets. In addition LunaScript RT SuperMix contains dNTPs and a blue tracking dye providing a visual indicator that can be followed throughout the two-step RT-qPCR process. This product offers robust linear and sensitive detection using total RNA inputs as high as 1 g and as low as single copies of RNA.
Biochain Institute Inc dATP, dCTP, dGTP, dTTP, 4x 100 ul (100 mM)/PK
Polymerase Chain Reaction (PCR) has become one of the most important technologies in the scientific community since its introduction in 1985. BioChain provides the most common used qPCR and PCR enzyme and reagents, such as qPCR SuperMix, Taq polymerase, dNTP, primers, at the most competitive price. This product line offers our customers of genomic DNA, RNA, and cDNA, a convenient and economic source for large quantity PCR enzymes and reagents.
Enzo Life Sciences AMPIPROBE HCV assay kit (100tests)
The AMPIPROBE HCV assay kit is a quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay for the quantitative detection of human hepatitis C virus (HCV) RNA in plasma or serum. The proprietary primer mix included in the kit is specific for HCV genotypes 1 through 6. The kit includes titered high, medium, and low HCV controls which enable virus quantitation in IU/mL. In addition, the kit contains an extraction control to ensure proper sample nucleic acid extraction. Enzo's AMPIPROBE technology incorporates probe detection technology in primer design. It employs a combination of fluorescent reporter-labeled primers and quencher-labeled primers to amplify DNA, akin to traditional PCR. When free in solution, fluorescent primers generate a signal. Applications: qPCR. Species reactivity: Hepatitis C virus.
XpressBio XpressMyco qPCR Mycoplasma Detection Kit, 100 Reactions
The XpressMyco qPCR Mycoplasma Detection Kit is a real-time PCR (qPCR) assay for rapid, quantitative detection of mycoplasma contaminants in cell culture.